Description
Practical Liquid Chromatography
1: Liquid ChromatographyThe Background. - 1. 1. Perspective. - 1. 2. Plan of the Book. - 1. 3. Chromatography and Its Basic Variants. - 1. 4. Development Methods in Chromatography. - 1. 5. Basic Experimental Arrangements. - 1. 6. Measures of the Effectiveness of Chromatographic Separations. - 1. 7. Retention Parameters. - References. - Further Reading. - 2: Band Spreading in Chromatography. - 2. 1. Introduction. - 2. 2. Factors Leading to Band Spreading. - 2. 3. The Random-Walk Concept. - 2. 4. Simple Mathematics of the Random Walk. - 2. 5. Molecular Diffusion. - 2. 6. Mass Transfer. - 2. 7. Eddy Diffusion. - 2. 8. Final Expression for Plate Height. - 2. 9. Extrabed Contributions to Band Spreading. - 2. 10. Columns versus Plates. - 2. 11. SummaryImportant Practical Consequences of Theory. - References. - Further Reading. - 3: Adsorption Chromatography: Mechanism and Materials. - 3. 1. Introduction. - 3. 2. Adsorption Isotherms. - 3. 3. Nature of Adsorption Forces. - 3. 4. Choice of Chromatographie System in LSAC. - 3. 5. Variation of Adsorption Coefficient K with Solute Structure. - 3. 6. Individual Adsorbents. - References. - Further Reading. - 4: Liquid Partition ChromatographyMechanism and Materials. - 4. 1. Introduction. - 4. 2. Thermodynamics of Liquid-Liquid Partition. - 4. 3. Selection of Phase Pairs. - 4. 4. Support Materials. - 4. 5. Further Practical Considerations. - References. - Further Reading. - 5: Exclusion ChromatographyMechanism and Materials. - 5. 1. Introduction. - 5. 2. Peak Broadening in Exclusion Chromatography. - 5. 3. Theories of Exclusion Chromatography. - 5. 4. Stationary Phases for Exclusion Chromatography. - Appendix: Applications of Exclusion Chromatography. - References. - Further Reading. - 6: The Technique of Thin-Layer Chromatography. - 6. 1. Introduction. - 6. 2. Outline of Technique. - 6. 3. TheStationary Phase. - 6. 4. Preparation of TLC Plates. - 6. 5. Application of the Sample. - 6. 6. Choice of Mobile Phase. - 6. 7. Development of Chromatogram. - 6. 8. Reproducibility of RF Values. - 6. 9. Detection of Solutes. - 6. 10. Chromatographic Records. - 6. 11. Direct Quantitative Analysis on the Layer. - 6. 12. Removal of Solute from Adsorbent Layer. - 6. 13. Use of Ancillary Techniques. - 6. 14. Present Status and Future Outlook. - References. - Further Reading. - 7: Equipment for Column Chromatography. - 7. 1. Introduction. - 7. 2. Pneumatics. - 7. 3. Gauges. - 7. 4. Gradient Elution Supply. - 7. 5. Sample Injectors. - 7. 6. Columns and Fittings. - 7. 7. Grinders and Sizing Equipment. - 7. 8. Fraction Collectors. - 7. 9. Detectors. - Appendix: A Short Guide to Commercial Equipment Suitable for Column Liquid Chromatography. - References. - Further Reading. - 8: Current Status of Liquid Chromatography: Place among Family of Chromatographic Techniques. - References. Language: English
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Fruugo ID:
450592625-949694071
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ISBN:
9781468419375
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